This Article Full Text (PDF) All Versions of this Article: 1545109709331808v1 8/2/106    most recent References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Molina, R. M. Articles by Costa, S. C. B. Search for Related Content PubMed PubMed Citation Articles by Molina, R. M. Articles by Costa, S. C. B. Social Bookmarking                         What's this?

Prevalence of HIV-1 Subtypes in Brazilian Children With Perinatally Acquired Infection

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Anali G. Torina

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Keila Biffi

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Beatriz A. P. Bismara, MSC

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Dulcineia M. Albuquerque, PhD

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Paula D. Andrade, MSc

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Emanuel B. V. Anjos

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Adleia D. C. Toro, MD, PhD

Department of Pediatrics, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Marcos T. Nolasco, MD, PhD

Department of Pediatrics, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Maria M. S. Vilela, MD, PhD

Department of Pediatrics, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil

Sandra C. B. Costa, MD, PhD

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil, costa{at}fcm.unicamp.br

HIV-1 infection has increased among women in recent years. The HIV-1 env gene (structural gene) has the greatest variation in all the HIV gene regions. In this study, 58 samples from infants infected with HIV-1 via perinatal transmission were analyzed. All the 58 samples were submitted to Nested-polymerase chain reaction of the env gene region for posterior viral genotyping using EN 70 and EN 85 (first polymerase chain reaction) and EN 80 and EN 95 (second polymerase chain reaction) primers, with the product of the 682 base pair amplification. After Nested-polymerase chain reaction for genotyping, purification of the product, and direct sequencing in a MegaBace 1000 automatic sequencer, 56 genotypes were found in the 58 HIV-1-positive children of the study, where 47 (83.93%) were HIV-1 subtype B infected and 9 (16.07%) were HIV-1 subtype F1 infected. The results demonstrate the predominance of subtype B followed by subtype F in Southeast Brazil.

Key Words: HIV-1 • Nested-PCR • genotyping • perinatal transmission

This version was published on March 1, 2009

Journal of the International Association of Physicians in AIDS Care (JIAPAC), Vol. 8, No. 2, 106-112 (2009)
DOI: 10.1177/1545109709331808


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?